Question 5 • AFC AP Biology B2
DNA polymerase adds each incoming nucleotide to the free 3′-OH group at the end of the growing strand. This extends the strand in the 5′ to 3′ direction, so choice C, “DNA polymerase acts on the OH group of the existing strand to add nucleotides,” is best. A template is needed to determine the sequence, but that does not explain the direction of synthesis.
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